Purpose/Significance To investigate the microbiota-gut-brain axis mechanism of autism spectrum disorder(ASD)，and to provide a basis for identifying potential therapeutic targets. Method/Process ASD-related gut microbiome data are obtained from PubMed，while ASD brain tissue gene expression profiles are retrieved from GEO. Clinical bioinformatics methods are applied to per-form differential gene analysis and Kyoto encyclopedia of genes and genomes(KEGG)pathway enrichment analysis. By identifying path-ways significantly enriched in both microbial communities and genes，the bipartite interaction networks linking“microbiota-pathway” and“gene-pathway”are constructed. The top 10 most strongly associated microbiota-gene pairs are selected based on similarity scores， and their associations are further analyzed using non-literature-based knowledge discovery approaches. Result/Conclusion Several microbiota-gene pairs suggest that gut microbiota may indirectly influence gene expression by modulating neuroinflammation，oxidative stress，and energy metabolism，offering theoretical insights into the mechanisms underlying the microbiota-gut-brain axis in ASD and potential therapeutic targets.